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OBJECTIVE: To study the efficacy of the neuromuscular electrical stimulation combined with swallowing training on the patients with swallowing dysfunction following acute cerebral infarction.Method Totally 100 patients with swallowing dysfunction following acute cerebral infarction were randomly divided into the experimental group(50 patients)and the control group(50 patients).The patients in the experimental group were treated with neuromuscular electrical stimulation combined with swallowing training,while the patients in the control group were treated with routine care and swallowing training.The improvement in swallowing function,complications,quality of life and prognosis were compared between the two groups after treatment.RESULTS: After treatment,the evaluation of drinking water test,SSA score and GUSS score in the experimental group and the control group were significantly improved compared with those before treatment(P<0.05),and the improvement of the experimental group was significantly better than that of the control group(P<0.05).The white blood cell count,CRP value,mRS score at 90 days,and the incidence of aspiration pneumonia and malnutrition in the experimental group were significantly lower than those in the control group(P<0.05),and the albumin,pre-albumin,total lymphocyte count and SWAL-QOL score at the time of discharge were significantly higher than those in the control group(P<0.05).CONCLUSION: Neuromuscular electrical stimulation combined with swallowing training has better effect than pure swallowing training in the patients with swallowing dysfunction due to acute cerebral infarction;the complication incidence is lower,the quality of life is higher,and the prognosis is better.
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OBJECTIVE To observe the effect of Epimedium 95％ethanol elution section (E95EE) on endogenous metabolism in the urine of normal rats using methods of metabonomics, and to study whether differential expressions of biomarkers can influence different systems of the body along with various body-fluids-cycle distribution. METHODS SD rats were randomly divided into blank control group and E95EE group(10 rats per group). The rats of E95EE group were ig administered with E95EE 17.1 g · L-1, once daily, for 20 d, while those of normal control group were ig given an equal volume of saline. On the day of the final E95EE administration, the urine of 12 h was collected for analysis by UPLC-TOF/MS. RESULTS This study identified nine differential endogenous metabolites (3-sn-phos-phatidate, 5, 10-methenyltetrahydrofolate, l-1-phosphatidylethanolamine, 1-acyl-glycerone 3-phosphate, N-formimidoyl-l-glutamate, keto-oxaloacetate, sulfurous acid, formyl-N-acetyl-5-methoxykynurenamin and N-acetyl-5-hydroxytryptamine) and six primary metabolic pathways [glycerophospholipid metabolism, vitamin A (retinol) metabolism, histidine degradation, tryptophan metabolism, acetyl-CoA biosynthesis from citrate, glycolysis and gluconeogenesis]. The possible role of protection of E95EE discovered in the nervous and cardiovascular systems was displayed by the decreased level of 3-sn-phosphatidate and an increased level of N-formimidoyl-l-glutamate. However, the possible toxicity of E95EE on neoplastic prevention was achieved by reducing the level of l-1-phosphatidylethanolamine and 5,10-methenyl tetra-hydrofolate. CONCLUSION E95EE can produce both protection and toxicity on nervous, cardiovascular and immune systems, as well as on tumor-associated diseases. The mechanisms may be related to metabolic pathways of triglycerides, vitamin A, tryptophane and histidines.
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OBJECTIVE@#To improve DNA extraction from human epithelium.@*METHODS@#Chelex-100, Chelex-100 organic combined and Chelex-100 magnetism pearl combined methods were adopted according to the situation of each case.@*RESULTS@#With the stepwise extracting protocol, the trace amount of DNA was analyzed most efficiently and the success rate of DNA extracting was better improved.@*CONCLUSION@#The efficiency of DNA extraction from human epithelium could be greatly improved by modifying the extracting protocol such as purification and concentration based on previous results.